Immunological Activity of Cytochrome c

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Immunological activity of cytochrome c. 3. Enhancement of antibody detection and immune response initiation by cytochrome c polymers.

Antibodies which fail to precipitate with the monomeric homologous cytochromes c were elicited in rabbits by the conjugate of bovine y-globulin and cytochrome c (tuna and turkey proteins) or by free monomeric cytochrome c (kangaroo and, in one case, the horse protein). Such antibodies do form precipitates and fix complement when allowed to react with the polymerized homologous antigen. In contr...

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Immunological activity of cytochrome c. I. Precipitating antibodies to monomeric vertebrate cytochromes c.

The production of antibodies by rabbits in response to monomeric preparations of human, Macaca mulatta, and horse cytochromes c, incorporated in complete Freund’s adjuvant, is described. These antibodies precipitate with the monomeric homologous antigens, whereas no such precipitates occurred with antisera elicited by monomeric preparations of the kangaroo (Macropus canguru), turkey, and tuna p...

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Immunological Activity of Cytochrome c III. ENHANCEMENT OF ANTIBODY DETECTION AND IMMUNE RESPONSE INITIATION BY CYTO- CHROME c POLYMERS

Antibodies which fail to precipitate with the monomeric homologous cytochromes c were elicited in rabbits by the conjugate of bovine y-globulin and cytochrome c (tuna and turkey proteins) or by free monomeric cytochrome c (kangaroo and, in one case, the horse protein). Such antibodies do form precipitates and fix complement when allowed to react with the polymerized homologous antigen. In contr...

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Reduction and activity of cytochrome c in the cytochrome c-cytochrome aa3 complex.

Uncharged reductants, such as NNN'N'-tetramethyl-p-phenylenediamine and diaminodurene, reduce cytochrome c at both high and low ionic strength, unlike ascorbate, which is effective only at low ionic strength. The 'tightly bound' cytochrome c-cytochrome c oxidase complex, with 1 equiv. of cytochrome c per cytochrome aa3, can be prepared by simple mixing of the two component species. Its properti...

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Sulfite oxidase activity of cytochrome c: Role of hydrogen peroxide

In humans, sulfite is generated endogenously by the metabolism of sulfur containing amino acids such as methionine and cysteine. Sulfite is also formed from exposure to sulfur dioxide, one of the major environmental pollutants. Sulfite is used as an antioxidant and preservative in dried fruits, vegetables, and beverages such as wine. Sulfite is also used as a stabilizer in many drugs. Sulfite t...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1970

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)63273-9